Ultrasound modulates ion channel currents

Ultrasound modulates ion channel currents.

Transcranial focused ultrasound (US) has been demonstrated to stimulate neurons in animals and humans, but the mechanism of this effect is unknown. It has been hypothesized that US, a mechanical stimulus, may mediate cellular discharge by activating mechanosensitive ion channels embedded within cellular membranes. To test this hypothesis, we expressed potassium and sodium mechanosensitive ion c...

Three-Dimensional Simulation of Qeshm Channel Currents

Qeshm Channel is a shallow and narrow waterway located between Qeshm Island and the mainland in the vicinity of Hormuzgan Province, Islamic Republic of Iran. This channel is important because of its economic, industrial, fisheries and navigation role it plays as well as environmental issues it presents in the region. A prognostic study was performed to simulate currents in this channel, using C...

Optimal Estimation of Ion-Channel Kinetics from Macroscopic Currents

Markov modeling provides an effective approach for modeling ion channel kinetics. There are several search algorithms for global fitting of macroscopic or single-channel currents across different experimental conditions. Here we present a particle swarm optimization(PSO)-based approach which, when used in combination with golden section search (GSS), can fit macroscopic voltage responses with a...

Measurement of Ensemble TRPV1 Ion Channel Currents Using Droplet Bilayers

Electrophysiological characterization of ion channels is useful for elucidation of channel function as well as quantitative assessment of pharmaceutical effects on ion channel conductance. We used droplet bilayers to measure ensemble ion channel currents from membrane preparations made from TRPV1-expressing HEK cells. Conductance measurements showed rectification, activation by acid and capsaic...

Ion channel activity drives ion channel expression.